Application

Goat anti-Human Ig κ chain Antibody, HRP conjugate, Species Adsorbed detects level of Human Ig κ chain & has been published & validated for use in ELISA.

ELISA: 1:4,000-1:8,000.

Optimal working dilutions must be determined by the end user.

General description

Antibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and lambda light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified by affinity chromatography on pooled human Igs with κ light chains covalently linked to agarose. Liquid 50% glycerol/50% PBS, pH 7.4.

Specificity

Reacts with human kappa light chains. Absorbed for human myeloma proteins with λ light chains and mouse immunoglobulins.